产品名称
SEN15 Rabbit Polyclonal Antibody
基因名称
TSEN15 C1orf19 SEN15
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/116461
Human Swissprot No.
Q8WW01
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q8WW01/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/66637
Mouse Swissprot No.
Q8R3W5
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8R3W5
免疫原
Synthesized peptide derived from human SEN15 AA range: 84-134
特异性
This antibody detects endogenous levels of SEN15 at Human/Mouse
稀释度
WB 1:500-2000;IHC-p 1:50-300
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from tRNA precursors. Alternative splicing results in multiple transcript variants. There is a pseudogene of this gene on chromosome 17. [provided by RefSeq, Jul 2014],
组织表达
Widely expressed. Highly expressed in testis and uterus.
细胞定位
Nucleus . Nucleus, nucleolus . May be transiently localized in the nucleolus. .
功能
caution:Although only weakly related to the S.cerevisiae SEN15 protein, it probably displays the same function within the tRNA splicing endonuclease complex.,function:Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3' end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3' end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.,similarity:Belongs to the SEN15 family.,subcellular location:May be transiently localized in the nucleolus.,subunit:tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3' end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2.,tissue specificity:Widely expressed. Highly expressed in testis and uterus.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
