产品名称
DNM3A Rabbit Polyclonal Antibody
蛋白名称
DNA (cytosine-5)-methyltransferase 3A (Dnmt3a) (EC 2.1.1.37) (DNA methyltransferase HsaIIIA) (DNA MTase HsaIIIA) (M.HsaIIIA)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9Y6K1
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9Y6K1/entry
Mouse Swissprot No.
O88508
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O88508
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q1LZ53
免疫原
Synthesized peptide derived from human protein . at AA range: 10-90
特异性
DNM3A Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016],
组织表达
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
细胞定位
Nucleus . Chromosome . Cytoplasm . Accumulates in the major satellite repeats at pericentric heterochromatin. .
信号通路
Cysteine and methionine metabolism;
功能
catalytic activity:S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.,caution:It is uncertain whether Met-1 or Met-4 is the initiator.,function:Required for genome wide de novo methylation and is essential for development. DNA methylation is coordinated with methylation of histones.,similarity:Belongs to the C5-methyltransferase family.,similarity:Contains 1 ADD-type zinc finger.,similarity:Contains 1 PWWP domain.,subunit:Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD-type zinc-finger (By similarity). Interacts with DNMT1 and DNMT3B. Interacts with the PRC2/EED-EZH2 complex.,tissue specificity:Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.