产品名称
LR16A Rabbit Polyclonal Antibody
基因名称
LRRC16A CARMIL LRRC16
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/55604
Human Swissprot No.
Q5VZK9
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q5VZK9/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/68732
Mouse Swissprot No.
Q6EDY6
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q6EDY6
免疫原
Synthesized peptide derived from human LR16A AA range: 853-903
特异性
This antibody detects endogenous levels of LR16A at Human/Mouse
稀释度
WB 1:500-2000;IHC-p 1:50-300
宿主
Polyclonal, Rabbit,IgG
组织表达
Expressed in lung, placenta, small intestine, liver, thymus, colon, skeletal muscle, heart and brain. Higher expression in kidney.
细胞定位
Cytoplasm . Cytoplasm, cytoskeleton . Cell membrane . Cell projection, lamellipodium . Found on macropinosomes (PubMed:19846667). Colocalized with heterodimeric capping protein (CP) and F-actin in lamellipodia but not with F-actin in stress fibers (PubMed:19846667). .
功能
function:Binds CAPZA2 with high affinity and significantly decreases CAPZA2 affinity for actin barbed ends. Increases the rate of elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Rapidly uncaps barbed ends capped by CAPZA2 and enhances barbed-end actin polymerization.,similarity:Belongs to the CARMIL family.,similarity:Contains 11 LRR (leucine-rich) repeats.,subcellular location:Colocalized with F-actin in lamellipodia but not with F-actin in stress fibers.,subunit:Interacts via its C-terminus with CAPZA2 in cytoplasm; interaction is essential for lamellipodial protrusion and cell translocation.,tissue specificity:Expressed in lung, placenta, small intestine, liver, thymus, colon, skeletal muscle, heart and brain. Higher expression in kidney.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
