产品名称
P4HA1 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/5033
Human Swissprot No.
P13674
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P13674/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/18451
Mouse Swissprot No.
Q60715
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q60715
Rat Gene Link
https://www.uniprot.org/uniprot/64475
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P54001
免疫原
Synthesized peptide derived from human P4HA1 AA range: 461-511
特异性
This antibody detects endogenous levels of P4HA1 at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
组织表达
Expressed in the heart, liver, skeletal muscle, kidney, placenta, lung and pancreas.
细胞定位
Endoplasmic reticulum lumen.
功能
catalytic activity:Procollagen L-proline + 2-oxoglutarate + O(2) = procollagen trans-4-hydroxy-L-proline + succinate + CO(2).,cofactor:Ascorbate.,cofactor:Binds 1 Fe(2+) ion per subunit.,function:Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.,similarity:Belongs to the P4HA family.,similarity:Contains 1 PKHD (prolyl/lysyl hydroxylase) domain.,similarity:Contains 1 TPR repeat.,subunit:Heterotetramer of two alpha-1 chains and two beta chains (the beta chain is the multi-functional PDI).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
