产品名称
FBLI1 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/54751
Human Swissprot No.
Q8WUP2
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q8WUP2/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/74202
Mouse Swissprot No.
Q71FD7
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q71FD7
免疫原
Synthesized peptide derived from human FBLI1 AA range: 157-207
特异性
This antibody detects endogenous levels of FBLI1 at Human/Mouse
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
组织表达
Isoform 1 and isoform 3 are expressed in heart, kidney, lung, pancreas, placenta and platelets. Isoform 2 is expressed in brain, heart, kidney, lung, pancreas, placenta, skeletal muscle and platelets.
细胞定位
Cell junction, focal adhesion . Cytoplasm, cytoskeleton, stress fiber . Associated with actin stress fiber at cell-ECM focal adhesion sites (PubMed:12679033, PubMed:18829455). Isoform 1 and isoform 3 are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with FERMT2 (PubMed:12679033). Isoform 2 is localized at actin stress fibers (PubMed:12496242). .
功能
function:Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin-containing signaling complexes that control actin assembly.,similarity:Contains 3 LIM zinc-binding domains.,subcellular location:Associated with actin stress fiber at cell-ECM focal adhesion sites. Isoform 1 and isoform 3 are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with PLEKHC1. Isoform 2 is localized at actin stress fibers.,subunit:Isoform 1 and isoform 3 interact with PLEKHC1, FLNA and FLNC. Isoform 2 interacts with FLNB. Interacts with NKX2-5.,tissue specificity:Isoform 1 and isoform 3 are expressed in heart, kidney, lung, pancreas, placenta and platelets. Isoform 2 is expressed in brain, heart, kidney, lung, pancreas, placenta, skeletal muscle and platelets.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
