产品名称
IBTK Rabbit Polyclonal Antibody
蛋白名称
Inhibitor of Bruton tyrosine kinase (IBtk)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9P2D0
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9P2D0/entry
Mouse Swissprot No.
Q6ZPR6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q6ZPR6
免疫原
Synthesized peptide derived from human protein . at AA range: 410-490
特异性
IBTK Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Bruton tyrosine kinase (BTK) is a protein tyrosine kinase that is expressed in B cells, macrophages, and neutrophils. The protein encoded by this gene binds to BTK and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. This gene has a pseudogene on chromosome 18. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014],
组织表达
Expressed in DeFew, HEK293T, HeLa and in Jurkat, MC3 and NB4 lymphoid cells (at protein level). Isoform 1 is the predominant isoform expressed in all examined tissues and cell lines. Highly expressed in hemopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes and bone marrow). Weakly or not expressed in other tissues.
细胞定位
Cytoplasm. Membrane; Peripheral membrane protein. Translocates to the plasma membrane upon IgM stimulation.; [Isoform 2]: Nucleus.
功能
function:Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription.,similarity:Contains 2 BTB (POZ) domains.,similarity:Contains 3 ANK repeats.,similarity:Contains 3 RCC1 repeats.,subcellular location:Translocates to the plasma membrane upon IgM stimulation.,subunit:Interacts with the PH domain of BTK.,tissue specificity:Highly expressed in hemopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes and bone marrow). Weakly or not expressed in other tissues.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
