BD-PT7341

CLCN3 Rabbit Polyclonal Antibody

常规抗体

CLCN3

CLCN3

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1182

https://www.uniprot.org/uniprot/1182

P51790

https://www.uniprot.org/uniprotkb/P51790/entry

12725

https://www.uniprot.org/uniprot/12725

P51791

https://www.uniprot.org/uniprotkb/P51791

84360

https://www.uniprot.org/uniprot/84360

P51792

https://www.uniprot.org/uniprotkb/P51792

Synthesized peptide derived from human CLCN3 AA range: 597-647

This antibody detects endogenous levels of CLCN3 at Human/Mouse/Rat

WB 1：500-2000

90kD

-20°C/1 year

Polyclonal, Rabbit,IgG

This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011],

Expressed primarily in tissues derived from neuroectoderm. Within the brain, its expression is particularly evident in the hippocampus, olfactory cortex, and olfactory bulb. Highly expressed in aortic and coronary vascular smooth muscle cells, and aortic endothelial cells. Also expressed in tracheal and alveolar epithelial cells, and intima and media of the pulmonary vessels. Expressed in bronchus and colon (at protein level).

[Isoform 1]: Early endosome membrane ; Multi-pass membrane protein . Late endosome membrane ; Multi-pass membrane protein . Lysosome membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Isoform 1 is localized mainly in late endosomes. .; [Isoform 2]: Golgi apparatus membrane ; Multi-pass membrane protein . Cell projection, ruffle membrane ; Multi-pass membrane protein . Isoform 2 is mainly enriched in the Golgi (PubMed:12471024). Colocalizes with SLC9A3R1/EBP50 in membrane ruffles (PubMed:11967229). .

domain:Isoform 2 contains a C-terminal PDZ-binding motif mediating the interaction with GOPC.,function:Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.,miscellaneous:The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters.,PTM:N-glycosylated.,similarity:Belongs to the chloride channel (TC 2.A.49) family.,similarity:Contains 2 CBS domains.,subcellular location:Isoform 1 is localized mainly in early and late endosomes.,subcellular location:Isoform 2 partially colocalized with isoform 1 but is mainly enriched in the Golgi.,subunit:Homo- or heterodimer. Isoform 2 interacts with GOPC, PDZK1 and SLC9A3R1/EBP50.,tissue specificity:Expressed primarily in tissues derived from neuroectoderm. Within the brain, its expression is particularly evident in the hippocampus, olfactory cortex, and olfactory bulb. Highly expressed in aortic and coronary vascular smooth muscle cells, and aortic endothelial cells. Also expressed in tracheal and alveolar epithelial cells, and intima and media of the pulmonary vessels. Expressed in bronchus and colon (at protein level).,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.