产品名称
CPSF5 Rabbit Polyclonal Antibody
基因名称
NUDT21 CFIM25 CPSF25 CPSF5
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/11051
Human Swissprot No.
O43809
Human Swissprot Link
https://www.uniprot.org/uniprotkb/O43809/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/68219
Mouse Swissprot No.
Q9CQF3
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9CQF3
Rat Gene Link
https://www.uniprot.org/uniprot/291877
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q4KM65
免疫原
Synthesized peptide derived from human CPSF5 AA range: 25-75
特异性
This antibody detects endogenous levels of CPSF5 at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides. [provided by RefSeq, Jul 2008],
组织表达
Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
细胞定位
Nucleus . Cytoplasm . Shuttles between the nucleus and the cytoplasm in a transcription- and XPO1/CRM1-independent manner, most probably in complex with the cleavage factor Im complex (CFIm) (PubMed:19864460). In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles (PubMed:15169763). .
功能
function:Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.,miscellaneous:Has no hydrolase activity as missing metal-binding sites in NUDIX motif.,PTM:Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 AND SIRT2.,similarity:Belongs to the Nudix hydrolase family. CPSF5 subfamily.,similarity:Contains 1 nudix hydrolase domain.,subcellular location:In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.,subunit:Homodimer. Component of the cleavage factor Im (CFIm) complex, composed of, at least, NUDT21/CPSF5 and CPSF6 or CPSF7. Interacts with CPSF6, PABPN1 and SNRNP70. Interacts with PAPOLA; the interaction is diminished by acteylation.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
