产品名称
M21D1 Rabbit Polyclonal Antibody
基因名称
MB21D1 cGAS C6orf150
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/115004
Human Swissprot No.
Q8N884
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q8N884/entry
免疫原
Synthesized peptide derived from human M21D1 AA range: 35-85
特异性
This antibody detects endogenous levels of M21D1 at Human
宿主
Polyclonal, Rabbit,IgG
组织表达
Expressed in the monocytic cell line THP1.
细胞定位
Nucleus . Chromosome . Cell membrane ; Peripheral membrane protein . Cytoplasm, cytosol . Mainly localizes in the nucleus, and at low level in the cytosol (PubMed:31808743, PubMed:31544964). On chromosomes, enriched on centromeric satellite and LINE DNA repeat elements (PubMed:30811988). Exported from the nucleus to the cytosol in a XPO1/CRM1 via the nuclear export signal in response to DNA stimulation (PubMed:33406424). Outside the nucleus, localizes at the cell membrane as a peripheral membrane protein in resting conditions: association to the cell membrane is mediated via binding to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) (PubMed:30827685). Localization at the cell membrane is required to limit the recognition of self-DNA (PubMed:30827685). Following detection of double-stranded DNA (dsDNA), released from the cell membrane into the cytosol in order to signal (PubMed:30827685). Upon transfection with dsDNA forms punctate structures that co-localize with DNA and Beclin-1 (BECN1) (PubMed:26048138). Phosphorylation at Tyr-215 promotes cytosolic retention (PubMed:30356214). In response to translation stress, translocates to the cytosol and associates with collided ribosomes (PubMed:34111399). .; (Microbial infection) Upon infection with virulent M.tuberculosis forms aggregates with dsDNA, non-virulent bacteria (without the ESX-1 locus) do not form these aggregates (PubMed:26048138). .
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
