产品名称
PHF5A Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/84844
Human Swissprot No.
Q7RTV0
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q7RTV0/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/68479
Mouse Swissprot No.
P83870
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P83870
Rat Gene Link
https://www.uniprot.org/uniprot/192246
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P83871
免疫原
Synthesized peptide derived from human PHF5A AA range: 33-83
特异性
This antibody detects endogenous levels of PHF5A at Human/Mouse/Rat
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes. [provided by RefSeq, Jul 2008],
细胞定位
Nucleus . Nucleus speckle .
功能
function:Acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER-alpha. Also involved in pre-mRNA splicing.,similarity:Belongs to the PHF5 family.,subunit:Component of splicing factor SF3B which is composed of at least eight subunits; SF3B1/SAP155/SF3B155, SF3B2/SAP145/SF3B145, SF3B3/SAP130/SF3B130, SF3B4/SAP49/SF3B49, SF3B14A, PHF5A/SF3B14B, SF3B10 and SF3B125. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
