产品名称
ZW10 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/9183
Human Swissprot No.
O43264
Human Swissprot Link
https://www.uniprot.org/uniprotkb/O43264/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/26951
Mouse Swissprot No.
O54692
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/O54692
Rat Gene Link
https://www.uniprot.org/uniprot/363059
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q4V8C2
免疫原
Synthesized peptide derived from human ZW10 AA range: 38-88
特异性
This antibody detects endogenous levels of ZW10 at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. This protein is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. [provided by RefSeq, Aug 2011],
细胞定位
Cytoplasm . Endoplasmic reticulum membrane ; Peripheral membrane protein . Chromosome, centromere, kinetochore . Cytoplasm, cytoskeleton, spindle . Lipid droplet . Dynamic pattern of localization during the cell cycle. In most cells at interphase, present diffusely in the cytoplasm (PubMed:15029241). In prometaphase, associated with the kinetochore. At metaphase, detected both at the kinetochores and, most prominently, at the spindle, particularly at the spindle poles. In very early anaphase, detected on segregating kinetochores. In late anaphase and telophase, accumulates at the spindle midzone (PubMed:11590237). .
功能
developmental stage:No significant variation in expression during cell cycle.,function:Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum.,miscellaneous:Overexpression as well as silencing of ZW10 disrupts the morphology of the ER-Golgi intermediate compartment as well as the Golgi apparatus and slows down ER-Golgi transport.,similarity:Belongs to the ZW10 family.,subcellular location:Dynamic pattern of localization during the cell cycle. In most cells at interphase, present diffusely in the cytoplasm. In prometaphase, associated with the kinetochore. At metaphase, detected both at the kinetochores and, most prominently, at the spindle, particularly at the spindle poles. In very early anaphase, detected on segregating kinetochores. In late anaphase and telophase, accumulates at the spindle midzone.,subunit:Associated with a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L, and SEC22B through direct interaction with RINT1/TIP20L bound to BNIP1/SEC20L. Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Interacts with C19orf25, KNTC1, and ZWINT.,tissue specificity:Widely expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
