产品名称
SHPRH Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/257218
Human Swissprot No.
Q149N8
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q149N8/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/268281
Mouse Swissprot No.
Q7TPQ3
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q7TPQ3
免疫原
Synthesized peptide derived from human SHPRH AA range: 9-59
特异性
This antibody detects endogenous levels of SHPRH at Human/Mouse
稀释度
WB 1:500-2000; IF ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed 17108083]).[supplied by OMIM, Mar 2008],
功能
domain:The RING finger mediates E3 ubiquitin ligase activity.,function:E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the SNF2/RAD54 helicase family.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 1 histone H1 domain.,similarity:Contains 1 PHD-type zinc finger.,similarity:Contains 1 RING-type zinc finger.,subunit:Homodimer. Interacts with PCNA, UBE2N and RAD18.,tissue specificity:Broadly expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
