产品名称
NEDD4 Rabbit Polyclonal Antibody
基因名称
NEDD4 KIAA0093 NEDD4-1 PIG53
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/4734
Human Swissprot No.
P46934
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P46934/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/17999
Mouse Swissprot No.
P46935
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P46935
Rat Gene Link
https://www.uniprot.org/uniprot/25489
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q62940
免疫原
Synthesized peptide derived from human NEDD4 AA range: 80-130
特异性
This antibody detects endogenous levels of NEDD4 at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016],
细胞定位
Cytoplasm . Cell membrane ; Peripheral membrane protein . Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1. .
功能
domain:The WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2.,function:E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). Involved in the budding of many viruses.,miscellaneous:A cysteine residue is required for ubiquitin-thioester formation.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 C2 domain.,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.,similarity:Contains 4 WW domains.,subunit:Interacts with UBE2D2. Binds SCNN1A, SCNN1B and SCNN1G. Binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif. Interacts with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2 (By similarity). Interacts with viral proteins that contain a late-budding motif P-P-P-Y. This interaction is essential for viral particle budding of a lot of retroviruses, like HTLV-1 Gag and MLV Gag.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
