产品名称
PRDX4 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/10549
Human Swissprot No.
Q13162
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q13162/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/53381
Mouse Swissprot No.
O08807
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/O08807
Rat Gene Link
https://www.uniprot.org/uniprot/85274
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q9Z0V5
免疫原
Synthesized peptide derived from human PRDX4 AA range: 112-162
特异性
This antibody detects endogenous levels of PRDX4 at Human/Mouse/Rat
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq, Jul 2008],
细胞定位
Cytoplasm . Endoplasmic reticulum . Cotranslationally translocated to and retained within the endoplasmic reticulum. A small fraction of the protein is cytoplasmic. .
功能
catalytic activity:2 R'-SH + ROOH = R'-S-S-R' + H(2)O + ROH.,function:Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.,miscellaneous:Irreversibly inactivated by overoxidation of Cys-124 (to Cys-SO(3)H) upon oxidative stress.,miscellaneous:The active site is the redox-active Cys-124 oxidized to Cys-SOH. Cys-SOH rapidly reacts with Cys-245-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin.,similarity:Belongs to the ahpC/TSA family.,similarity:Contains 1 thioredoxin domain.,subunit:Homodimer or heterodimer with PRDX1; disulfide-linked, upon oxidation.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
