产品名称
PUS10 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/150962
Human Swissprot No.
Q3MIT2
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q3MIT2/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/74467
Mouse Swissprot No.
Q9D3U0
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9D3U0
免疫原
Synthesized peptide derived from human PUS10 AA range: 425-475
特异性
This antibody detects endogenous levels of PUS10 at Human/Mouse
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009],
细胞定位
Nucleus . Cytoplasm . Mitochondrion . Localizes mainly in the nucleus (Probable) (PubMed:31819270). tRNA pseudouridylate synthase activity is restricted to the cytoplasm (PubMed:31819270). Translocates from nucleus to mitochondria during TRAIL-induced apoptosis (PubMed:28981101). .
功能
catalytic activity:tRNA uridine = tRNA pseudouridine.,function:Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Partially unspliced pre-RNA.,similarity:Belongs to the pseudouridine synthase Pus10 family.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
