产品名称
LCAT Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/3931
Human Swissprot No.
P04180
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P04180/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/16816
Mouse Swissprot No.
P16301
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P16301
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P18424
免疫原
Synthesized peptide derived from human LCAT AA range: 6-56
特异性
This antibody detects endogenous levels of LCAT at Human/Mouse/Rat
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008],
组织表达
Detected in blood plasma (PubMed:3458198, PubMed:8820107, PubMed:10222237). Detected in cerebral spinal fluid (at protein level) (PubMed:10222237). Detected in liver (PubMed:3797244, PubMed:3458198). Expressed mainly in brain, liver and testes.
细胞定位
Secreted . Secreted into blood plasma (PubMed:3458198, PubMed:8820107, PubMed:10222237). Produced in astrocytes and secreted into cerebral spinal fluid (CSF) (PubMed:10222237). .
功能
catalytic activity:Phosphatidylcholine + a sterol = 1-acylglycerophosphocholine + a sterol ester.,disease:Defects in LCAT are a cause of fish-eye disease (FED) [MIM:136120]; also known as dyslipoproteinemic corneal dystrophy or alpha-LCAT deficiency. FED is due to a partial LCAT deficiency that affects only alpha-LCAT activity. It is characterized by low plasma HDL and corneal opacities due to accumulation of cholesterol deposits in the cornea ('fish-eye').,disease:Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) [MIM:245900]; also called Norum disease. LCATD is a disorder of lipoprotein metabolism characterized by inadequate esterification of plasmatic cholesterol. Two clinical forms are recognized: familial LCAT deficiency and fish-eye disease. Familial LCAT deficiency is associated with a complete absence of alpha and beta LCAT activities and results in esterification anomalies involving both HDL (alpha-LCAT activity) and LDL (beta-LCAT activity). It causes a typical triad of diffuse corneal opacities, target cell hemolytic anemia, and proteinuria with renal failure.,enzyme regulation:Apolipoprotein A-I is a potent activator for this enzyme.,function:Central enzyme in the extracellular metabolism of plasma lipoproteins. Among other substrates it esterifies the free cholesterol transported in plasma lipoproteins.,online information:Lecithin-cholesterol acyltransferase entry,similarity:Belongs to the AB hydrolase superfamily. Lipase family.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
