产品名称
APEX2 Rabbit Polyclonal Antibody
基因名称
APEX2 APE2 APEXL2 XTH2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/27301
Human Swissprot No.
Q9UBZ4
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9UBZ4/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/77622
Mouse Swissprot No.
Q68G58
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q68G58
免疫原
Synthesized peptide derived from human APEX2 AA range: 196-246
特异性
This antibody detects endogenous levels of APEX2 at Human/Mouse
宿主
Polyclonal, Rabbit,IgG
背景介绍
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012],
组织表达
Highly expressed in brain and kidney. Weakly expressed in the fetal brain.
细胞定位
Nucleus. Cytoplasm. Mitochondrion . Together with PCNA, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
功能
catalytic activity:The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.,function:May participate in both nuclear and mitochondrial post-replicative base excision repair (BER). In the nucleus functions in the PCNA-dependent BER pathway.,similarity:Belongs to the DNA repair enzymes AP/exoA family.,subcellular location:Colocalized partly with PCNA in nuclear foci.,subunit:Interacts with PCNA. This interaction is increased by misincorporation of uracil in nuclear DNA.,tissue specificity:Highly expressed in cells, adult brain and kidney. Weakly expressed in the fetal brain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
