产品名称
PLCD3 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/113026
Human Swissprot No.
Q8N3E9
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q8N3E9/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/72469
Mouse Swissprot No.
Q8K2J0
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8K2J0
免疫原
Synthesized peptide derived from human PLCD3 AA range: 501-551
特异性
This antibody detects endogenous levels of PLCD3 at Human/Mouse
稀释度
WB 1:500-2000;IHC-p 1:50-300
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids. [provided by RefSeq, Jul 2008],
组织表达
Present in corneal epithelial cells (at protein level).
细胞定位
Membrane; Peripheral membrane protein. Cytoplasm. Cleavage furrow . Localizes at the cleavage furrow during cytokinesis. .
功能
catalytic activity:1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.,cofactor:Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain.,domain:The C2 domain is a Ca(2+)-dependent membrane-targeting module.,domain:The PH domain mediates interaction with the surface membrane by binding to PIP2.,enzyme regulation:Strongly activated by phosphatidic acid. Inhibited by phosphatidylethanolamine (PtdEtn), phosphatidylcholine (PtdCho), sphingomyelin and phosphatidylserine (PtdSer).,function:Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Essential for trophoblast and placental development. May participate in cytokinesis by hydrolyzing PIP2 at the cleavage furrow.,induction:Down-regulated by Ca(2+) and cAMP.,similarity:Contains 1 C2 domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 PI-PLC X-box domain.,similarity:Contains 1 PI-PLC Y-box domain.,similarity:Contains 3 EF-hand domains.,subcellular location:Localizes at the cleavage furrow during cytokinesis.,tissue specificity:Present in corneal epithelial cells (at protein level).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
