产品名称
P4HA3 Rabbit Polyclonal Antibody
基因名称
P4HA3 UNQ711/PRO1374
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/283208
Human Swissprot No.
Q7Z4N8
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q7Z4N8/entry
Mouse Swissprot No.
Q6W3F0
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q6W3F0
Rat Gene Link
https://www.uniprot.org/uniprot/361612
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q6W3E9
免疫原
Synthesized peptide derived from human P4HA3 AA range: 211-261
特异性
This antibody detects endogenous levels of P4HA3 at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],
组织表达
Highly expressed in placenta, liver and fetal skin. Weakly expressed in fetal epiphyseal cartilage, fetal liver, fibroblast, lung and skeletal muscle. Expressed also in fibrous cap of carotid atherosclerotic lesions.
细胞定位
Endoplasmic reticulum lumen .
功能
catalytic activity:Procollagen L-proline + 2-oxoglutarate + O(2) = procollagen trans-4-hydroxy-L-proline + succinate + CO(2).,cofactor:Ascorbate.,cofactor:Binds 1 Fe(2+) ion per subunit.,function:Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.,PTM:N-glycosylation plays no role in the catalytic activity.,similarity:Belongs to the P4HA family.,similarity:Contains 1 PKHD (prolyl/lysyl hydroxylase) domain.,similarity:Contains 1 TPR repeat.,subunit:Heterotetramer of two alpha-3 chains and two beta chains (the beta chain is the multi-functional PDI).,tissue specificity:Highly expressed in placenta, liver and fetal skin. Weakly expressed in fetal epiphyseal cartilage, fetal liver, fibroblast, lung and skeletal muscle. Expressed also in fibrous cap of carotid atherosclerotic lesions.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
