产品名称
PTRF Rabbit Polyclonal Antibody
蛋白名称
Polymerase I and transcript release factor (Cavin-1)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q6NZI2
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q6NZI2/entry
Mouse Swissprot No.
O54724
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O54724
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P85125
免疫原
Synthesized peptide derived from human protein . at AA range: 90-170
特异性
PTRF Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrop
组织表达
Adipocyte,Epithelium,Lung,Muscle,Pancreas,Testis,
细胞定位
Membrane, caveola . Cell membrane . Microsome . Endoplasmic reticulum . Cytoplasm, cytosol . Mitochondrion . Nucleus . Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Colocalizes with CAV1 in lipid rafts in adipocytes. Localizes in the caveolae in a caveolin-dependent manner (By similarity). .
功能
function:Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.,PTM:Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent.,PTM:Phosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity.,similarity:Belongs to the PTRF/SDPR family.,subcellular location:Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation.,subunit:Interacts with RNA polymerase I and TTF1. Binds the 3' end of pre-rRNA. Interacts with transcription factor ZNF148 (By similarity). Interacts with LIPE in the adipocyte cytoplasm.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
