产品名称
PNKP Rabbit Polyclonal Antibody
蛋白名称
Bifunctional polynucleotide phosphatase/kinase (DNA 5'-kinase/3'-phosphatase) (Polynucleotide kinase-3'-phosphatase) [Includes: Polynucleotide 3'-phosphatase (EC 3.1.3.32) (2'(3')-polynucleotidase); Polynucleotide 5'-hydroxyl-kinase (EC 2.7.1.78)]
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q96T60
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q96T60/entry
Mouse Swissprot No.
Q9JLV6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9JLV6
免疫原
Synthesized peptide derived from human protein . at AA range: 50-130
特异性
PNKP Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010],
组织表达
Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
细胞定位
Nucleus . Chromosome . Localizes to site of double-strand breaks. .
功能
catalytic activity:A 3'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate.,catalytic activity:ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.,function:Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:In the N-terminal section; belongs to the DNA 3' phosphatase family.,tissue specificity:Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
