产品名称
CRY2 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/1408
Human Swissprot No.
Q49AN0
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q49AN0/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/12953
Mouse Swissprot No.
Q9R194
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9R194
Rat Gene Link
https://www.uniprot.org/uniprot/170917
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q923I8
免疫原
Synthesized peptide derived from human CRY2 AA range: 181-231
特异性
This antibody detects endogenous levels of CRY2 at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014],
组织表达
Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle.
细胞定位
Cytoplasm . Nucleus . Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL.
功能
cofactor:Binds 1 5,10-methenyltetrahydrofolate non-covalently per subunit.,cofactor:Binds 1 FAD per subunit.,function:Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.,online information:Cryptochrome entry,PTM:Phosphorylation on Ser-266 by MAPK is important for the inhibition of CLOCK-ARNTL-mediated transcriptional activity. Phosphorylation by CSKNE requires interaction with PER1 or PER2.,similarity:Belongs to the DNA photolyase class-1 family.,similarity:Contains 1 DNA photolyase domain.,subcellular location:Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL.,subunit:Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with PER1 and PER2 C-terminal domains. Interaction with PER2 inhibits its ubiquitination and vice versa. Interacts with NFIL3.,tissue specificity:Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, sketal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
