产品名称
CLIC5 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/53405
Human Swissprot No.
Q9NZA1
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9NZA1/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/224796
Mouse Swissprot No.
Q8BXK9
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8BXK9
Rat Gene Link
https://www.uniprot.org/uniprot/94272
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q9EPT8
免疫原
Synthesized peptide derived from human CLIC5 AA range: 156-206
特异性
This antibody detects endogenous levels of CLIC5 at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the chloride intracellular channel (CLIC) family of chloride ion channels. The encoded protein associates with actin-based cytoskeletal structures and may play a role in multiple processes including hair cell stereocilia formation, myoblast proliferation and glomerular podocyte and endothelial cell maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011],
组织表达
Widely expressed in both fetal and adult human tissues (PubMed:24781754). Isoform 1 is expressed in renal glomeruli endothelial cells and podocytes (at protein level).
细胞定位
[Isoform 1]: Cytoplasm, cytoskeleton . Cytoplasm, cell cortex . Membrane ; Single-pass membrane protein . Apical cell membrane ; Single-pass membrane protein . Cytoplasm . Associates with the cortical actin cytoskeleton (PubMed:10793131, PubMed:15184393). Localizes to the apical region of cochlear hair cells, at the base of the actin-rich hair bundle (By similarity). Colocalizes with podocalyxin at the apical cell membrane in renal glomeruli (PubMed:20335315). May localize to the centrosome in lens epithelial cells (By similarity). Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (By similarity). .; [Isoform 2]: Golgi apparatus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Colocalizes with AKAP9 at the Golgi apparatus as well as, to a lesser extent, the centrosome. .
功能
domain:Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.,function:Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti.,similarity:Belongs to the chloride channel CLIC family.,similarity:Contains 1 GST C-terminal domain.,subcellular location:Associates with the cortical actin cytoskeleton. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.,subcellular location:Colocalized with AKAP9 at the Golgi apparatus as well as, to a lesser extent, the centrosome. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.,subunit:Component of a multimeric complex consisting of several cytoskeletal proteins, including actin, ezrin, alpha-actinin, gelsolin, and IQGAP1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
