产品名称
KTNB1 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/10300
Human Swissprot No.
Q9BVA0
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9BVA0/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/74187
Mouse Swissprot No.
Q8BG40
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8BG40
免疫原
Synthesized peptide derived from human KTNB1 AA range: 72-122
特异性
This antibody detects endogenous levels of KTNB1 at Human/Mouse
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family of ATPases. [provided by RefSeq, Jul 2008],
细胞定位
Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, spindle . Predominantly cytoplasmic. Localized to the interphase centrosome and mitotic spindle poles (PubMed:9658175). Localizes within the cytoplasm, partially overlapping with microtubules, in interphase and to the mitotic spindle and spindle poles during mitosis (PubMed:26929214). .
功能
function:Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.,similarity:Belongs to the WD repeat KATNB1 family.,similarity:Contains 6 WD repeats.,subcellular location:Predominantly cytoplasmic. Localized to the interphase centrosome and mitotic spindle poles.,subunit:Interacts with PAFAH1B1 (By similarity). Interacts with KATNA1. This interaction enhances the microtubule binding and severing activity of KATNA1 and also targets this activity to the centrosome. Interacts with dynein, microtubules and NDEL1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
