产品名称
AP2M1 Rabbit Polyclonal Antibody
基因名称
AP2M1 CLAPM1 KIAA0109
蛋白名称
AP-2 complex subunit mu (AP-2 mu chain) (Adapter-related protein complex 2 mu subunit) (Adaptin-mu2) (Adaptor protein complex AP-2 subunit mu) (Clathrin assembly protein complex 2 medium chain) (Clathrin coat assembly protein AP50) (Clathrin coat-associated protein AP50) (HA2 50 kDa subunit) (Plasma membrane adaptor AP-2 50 kDa protein)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q96CW1
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q96CW1/entry
Mouse Swissprot No.
P84091
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P84091
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P84092
免疫原
Synthesized peptide derived from human protein . at AA range: 100-180
特异性
AP2M1 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015],
组织表达
Expressed in the brain (at protein level).
细胞定位
Cell membrane . Membrane, coated pit ; Peripheral membrane protein; Cytoplasmic side. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. .
信号通路
Endocytosis;Huntington's disease;
功能
function:Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. AP50 is a subunit of the plasma membrane adaptor. The complex binds polyphosphoinositide-containing lipids.,PTM:Phosphorylated.,similarity:Belongs to the adaptor complexes medium subunit family.,similarity:Contains 1 MHD (mu homology) domain.,subcellular location:Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.,subunit:Adaptor protein complex 2 (AP-2) is an heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1). Interacts with ATP6V1H and MEGF10.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
