产品名称
LRWD1 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/222229
Human Swissprot No.
Q9UFC0
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9UFC0/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/71735
Mouse Swissprot No.
Q8BUI3
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8BUI3
免疫原
Synthesized peptide derived from human LRWD1 AA range: 395-445
特异性
This antibody detects endogenous levels of LRWD1 at Human/Mouse
稀释度
WB 1:500-2000;IHC-p 1:50-300
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene interacts with components of the origin recognition complex (ORC) and regulates the formation of the prereplicative complex. The encoded protein stabilizes the ORC and therefore aids in DNA replication. This protein is required for the G1/S phase transition of the cell cycle. In addition, the encoded protein binds to trimethylated histone H3 in heterochromatin and recruits the ORC and lysine methyltransferases, which help maintain the repressive heterochromatic state. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015],
组织表达
Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS).
细胞定位
Nucleus . Chromosome, centromere. Chromosome, telomere. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore . Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres. Recruitment to pericentric heterochromatin largely depends on the presence of H3K9me3.
功能
PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 3 LRR (leucine-rich) repeats.,similarity:Contains 5 WD repeats.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
