产品名称
UBE2B Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/7320
Human Swissprot No.
P63146
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P63146/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/22210
Mouse Swissprot No.
P63147
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P63147
Rat Gene Link
https://www.uniprot.org/uniprot/81816
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P63149
免疫原
Synthesized peptide derived from human UBE2B AA range: 23-73
特异性
This antibody detects endogenous levels of UBE2B at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008],
细胞定位
Cell membrane . Nucleus . In peripheral neurons, expressed both at the plasma membrane and in nuclei. .
功能
catalytic activity:ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.,function:Catalyzes the covalent attachment of ubiquitin to other proteins. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-conjugating enzyme family.,subcellular location:In peripheral neurons, expressed both at the plasma membrane and in nuclei.,subunit:Interacts with RAD18 and UBR2.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
