产品名称
CKAP5 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/9793
Human Swissprot No.
Q14008
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q14008/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/75786
Mouse Swissprot No.
A2AGT5
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/A2AGT5
免疫原
Synthesized peptide derived from human CKAP5 AA range: 1446-1496
特异性
This antibody detects endogenous levels of CKAP5 at Human/Mouse
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011],
组织表达
Overexpressed in hepatomas and colonic tumors. Also expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas. Expression is elevated in the brain; highly expressed in the Purkinje cell bodies of the cerebellum.
细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, spindle . Chromosome, centromere, kinetochore . Detected on centrosomes and kinetochores during interphase and mitosis independently from TACC3 and clathrin. Located to spindle poles and microtubules during mitosis. In complex with TACC3 localized to microtubule plus-ends in mitosis and interphase. In complex with TACC3 and clathrin localized to inter-microtubule bridges in mitotic spindles. Accumulation sites at microtubule plus ends protruded approximately 100 nm from MAPRE1/EB1 sites in interphase cells. .
功能
function:Plays a major role in organizing spindle poles.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the TOG/XMAP215 family.,similarity:Contains 10 HEAT repeats.,subunit:Interacts with TACC1.,tissue specificity:Over-expressed in hepatomas and colonic tumors. Also expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
