产品名称
CLSPN Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9HAW4
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9HAW4/entry
Mouse Swissprot No.
Q80YR7
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q80YR7
免疫原
Synthesized peptide derived from human protein . at AA range: 10-90
特异性
CLSPN Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The product of this gene is an essential upstream regulator of checkpoint kinase 1 and triggers a checkpoint arrest of the cell cycle in response to replicative stress or DNA damage. The protein is also required for efficient DNA replication during a normal S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010],
组织表达
Bone marrow,Epithelium,PCR rescued clones,Skin,
功能
domain:The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.,function:Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.,induction:Expression peaks at S/G2 phases of the cell cycle.,PTM:Phosphorylated during activation of DNA replication or DNA damage checkpoints. This requires ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the claspin family.,subunit:Interacts with CHEK1 when phosphorylated. Interacts with ATR and RAD9A and these interactions are slightly reduced during checkpoint activation. Interacts with BRCA1 and this interaction increases during checkpoint activation. Interacts with TIMELESS.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
